Myoglobin facilitates oxygen diffusion

In this study, the hemodynamic effects of acute myoglobin (Mb) inhibition with CO on isolated hearts of wild‐type (WT) mice were examined at different degrees of oxygenation. Hearts from myoglobin knockout (myo−/−) mice served as appropriate controls. The intracellular MbO2 dissociation curve, as me...

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Bibliographic Details
Published in:The FASEB journal 2001-04, Vol.15 (6), p.1077-1079
Main Authors: Merx, Marc W., Flögel, Ulrich, Stumpe, Thomas, Gödecke, Axel, Decking, Ulrich K. M., Schrader, Jürgen
Format: Article
Language:English
Subjects:
facilitated diffusion
ischemia
magnetic resonance spectroscopy
myoglobin knockout mouse
oxidative phosphorylation
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Summary:In this study, the hemodynamic effects of acute myoglobin (Mb) inhibition with CO on isolated hearts of wild‐type (WT) mice were examined at different degrees of oxygenation. Hearts from myoglobin knockout (myo−/−) mice served as appropriate controls. The intracellular MbO2 dissociation curve, as measured by 1H NMR, was determined by systematically lowering the O2 content of the perfusion medium. At 100% MbO2 saturation (buffer O2: 75%), complete inhibition of Mb with 20% CO did not alter left ventricular developed pressure (LVDP) or coronary venous PO2 (PvO2) and thus myocardial O2 consumption. At 87% MbO2 saturation (buffer O2: 65%), CO applied to WT hearts significantly decreased LVDP by 12% and increased PvO2 by 30% (both P
ISSN:0892-6638
1530-6860
DOI:10.1096/fsb2fj000497fje