Benzene-Induced Inhibition of Erythroid Colony Formation in Vitro

The erythroid colony forming unit assay technique was used to study the inhibitory effect of benzene on mouse bone marrow cell culture in vitro. The ability of vitamin B6 to prevent benzene-induced inhibition of erythroid colony formation was also tested. At concentrations equal to or higher than th...

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Bibliographic Details
Published in:The American journal of the medical sciences 1986-12, Vol.292 (6), p.356-362
Main Authors: Daudu, Peter A., Geelhoed, Glenn W.
Format: Article
Language:English
Subjects:
Anemia
Animals
Benzene - toxicity
Biological and medical sciences
Bone Marrow Toxicity
Cells, Cultured
Chemical and industrial products toxicology. Toxic occupational diseases
Colony-Forming Units Assay
Depression, Chemical
Dose-Response Relationship, Drug
Erythroblasts - drug effects
Hematopoietic Stem Cells - drug effects
Histological Techniques
In Vitro Techniques
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Pyridoxine
Pyridoxine - pharmacology
Solvents
Toxicology
Vitamin B6
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Summary:The erythroid colony forming unit assay technique was used to study the inhibitory effect of benzene on mouse bone marrow cell culture in vitro. The ability of vitamin B6 to prevent benzene-induced inhibition of erythroid colony formation was also tested. At concentrations equal to or higher than the lowest inhibitory concentration of benzene, pyridoxine HCl significantly prevented benzene-induced inhibition of erythroid colony formation in vitro. However, when benzene concentration was higher, the added pyridoxine HCl was ineffective in preventing benzene-induced inhibition of erythroid colony formation in vitro, even when it was preincubated with the bone marrow cells for 30 minutes prior to adding benzene. On the basis of this finding, it is proposed that benzene-induced inhibition of erythroid colony formation may involve two different mechanisms, one of which involves interaction with vitamin Bo added as pyridoxine HCI.
ISSN:0002-9629
1538-2990
DOI:10.1097/00000441-198612000-00004