Increased acute and chronic mitotic activity in rat laryngeal muscles after botulinum toxin injection

Objectives: To characterize the acute and chronic cellular effects of botulinum toxin (BT) injection into rat laryngeal muscles. A complete characterization of these effects is important because patients with focal dystonias of the head and neck are commonly treated with BT injection. Further, poten...

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Bibliographic Details
Published in:The Laryngoscope 1998-07, Vol.108 (7), p.1055-1061
Main Authors: Inagi, Katsuhide, Connor, Nadine P., Schultz, Edward, Ford, Charles N., Cook, Crist H., Bless, Diane M., Heisey, Dennis M.
Format: Article
Language:English
Subjects:
Animals
Anti-Dyskinesia Agents - pharmacology
Biological and medical sciences
Blepharospasm - drug therapy
botulinum toxin
Botulinum Toxins - pharmacology
BrdU
Bromodeoxyuridine
Cell Division - drug effects
Drug Administration Schedule
Drug Evaluation, Preclinical
Ent. Stomatology
Injections, Intramuscular
Laryngeal Muscles - cytology
Laryngeal Muscles - drug effects
larynx
Male
Medical sciences
Mitosis - drug effects
Pharmacology. Drug treatments
Rat
Rats
Rats, Sprague-Dawley
satellite cell
spasmodic dysphoria
Time Factors
Voice Disorders - drug therapy
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Summary:Objectives: To characterize the acute and chronic cellular effects of botulinum toxin (BT) injection into rat laryngeal muscles. A complete characterization of these effects is important because patients with focal dystonias of the head and neck are commonly treated with BT injection. Further, potential muscular changes in the larynx must be carefully delineated owing to the critical phonatory and airway protective functions of these muscles. Study Design: The acute and chronic cellular effects of BT injection were studied using 5′‐bromo 2′‐deoxyuridine (BrdU) following single and repeated BT injection into rat laryngeal muscles. BrdU is incorporated into mitotically active nuclei such that changes in cell proliferative behavior following BT injection can be monitored. Results: Increased mitotic activity was detected in the tissue samples studied following BT injection. Differences in the times of the peak distribution of BrdU‐labeled cells in each laryngeal muscle were observed. This may be related to the diffusion effects of BT. Prolonged muscle fiber changes, including splitting, were also observed as the result of repeated BT injection. Conclusions: The results of this study suggest that BT may induce a proliferative response in muscle tissue.
ISSN:0023-852X
1531-4995
DOI:10.1097/00005537-199807000-00019