Identification and functional characterization of dendritic cells in the healthy murine kidney and in experimental glomerulonephritis

The kidney tubulointerstitium contains numerous bone marrow-derived antigen-presenting cells, which are often referred to as resident tissue macrophages, although several previous studies had demonstrated characteristics of dendritic cells (DC). In this study, we describe a subset of tubulointerstit...

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Bibliographic Details
Published in:Journal of the American Society of Nephrology 2004-03, Vol.15 (3), p.613-621
Main Authors: KRÜGER, Thilo, BENKE, Dirk, KURTS, Christian, EITNER, Frank, LANG, Andreas, WIRTZ, Monika, HAMILTON-WILLIAMS, Emma E, ENGEL, Daniel, GIESE, Bernd, MÜLLER-NEWEN, Gerhard, FLOEGE, Jürgen
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
CD11b Antigen
CD11c Antigen
Dendritic Cells - physiology
Female
Glomerulonephritis
Glomerulonephritis - pathology
Kidney - cytology
Macrophages
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
Phenotype
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Summary:The kidney tubulointerstitium contains numerous bone marrow-derived antigen-presenting cells, which are often referred to as resident tissue macrophages, although several previous studies had demonstrated characteristics of dendritic cells (DC). In this study, we describe a subset of tubulointerstitial cells expressing the DC marker CD11c. A protocol was established to isolate these cells for in vitro analysis. Renal CD11c(+) cells resembled splenic DC, but not peritoneal macrophages, in morphology, lysosomal content, phagocytic activity, microbicidal effector functions, expression of T cell costimulatory molecules, and ability to activate T cells. Nevertheless, many CD11c(+) renal cells expressed low or intermediate levels of F4/80 and CD11b, indicating that both markers are not absolutely specific for macrophages in the kidney. Subpopulations of renal DC could be distinguished based on their expression of MHC class II and costimulatory molecules and may represent different maturation stages. In nephrotoxic glomerulonephritis, increased numbers of CD11c(+) cells showing DC functionality were found in the tubulointerstitium. Focal accumulation was seen within tubulointerstitial mononuclear infiltrates and adjacent to, but not within, inflamed glomeruli. These results are the first to identify and characterize renal CD11c(+) cells as DC and to demonstrate marked changes in experimental glomerulonephritis.
ISSN:1046-6673
1533-3450
DOI:10.1097/01.asn.0000114553.36258.91