Cyclic AMP signalling pathway and trehalase activation in the fission yeast Schizosaccharomyces pombe

Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, 30071 Murcia, Spain * Author for correspondence: Mariano Gacto. Tel: +34 68 307 100. Fax: +34 68 363 963. ABSTRACT The response of derepressed cells of Schizosaccharomyces pombe to the addition of glucose included a...

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Published in:Microbiology (Society for General Microbiology) 1994-06, Vol.140 (6), p.1467-1472
Main Authors: Carrillo, Dolores, Vicente-Soler, Jeronima, Gacto, Mariano
Format: Article
Language:English
Subjects:
2,4-Dinitrophenol
Azides - pharmacology
Biological and medical sciences
Cyclic AMP - physiology
Cycloheximide - pharmacology
Dinitrophenols - pharmacology
Enzyme Activation - physiology
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Microbiology
Mycology
Schizosaccharomyces - enzymology
Schizosaccharomyces - physiology
Second Messenger Systems
Sodium Azide
Temperature
Trehalase - metabolism
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Summary:Department of Genetics and Microbiology, Facultad de Biologia, University of Murcia, 30071 Murcia, Spain * Author for correspondence: Mariano Gacto. Tel: +34 68 307 100. Fax: +34 68 363 963. ABSTRACT The response of derepressed cells of Schizosaccharomyces pombe to the addition of glucose included a marked and reversible activation of neutral trehalase that was not produced in repressed cells. The protein synthesis inhibitor cycloheximide, the protonophore 2,4-dinitrophenol or the uncoupler sodium azide also enhanced trehalase activity in derepressed cells provided glucose was present in the incubation assays. However, only 2,4-dinitrophenol or cycloheximide was able to induce trehalase activation in repressed cells. Stimulation of trehalase by these compounds was preceded in all cases by a rapid increase in adenosine 3'-5'-cyclic monophosphate (cAMP) content. Since exogenous cAMP can activate trehalase both in repressed and derepressed growing cells, the results provide evidence for the existence of an induced cAMP signalling pathway in the fission yeast with several entries for trehalase activation. The correlation between cAMP increase and trehalase activation was not maintained when the enzyme was heat-shock-activated, supporting the concept that trehalase activity can be also enhanced in cells by another mechanism in which cAMP does not act as second messenger. Keywords: Schizosaccharomyces pombe, trehalase activation, cyclic AMP, signalling pathways
ISSN:1350-0872
1465-2080
DOI:10.1099/00221287-140-6-1467