Inactivation of Southern Bean Mosaic Virus and its Ribonucleic Acid by Nitrous Acid and Ultraviolet Light

• Published in: Journal of general virology 1973-01, Vol.18 (1), p.1-10
• Main Author: Sehgal, O. P
• Format: Article
• Language: English
• Subjects: Centrifugation, Density Gradient; Deamination; Electrophoresis; Immunodiffusion; Light; Mosaic Viruses - drug effects; Mosaic Viruses - isolation & purification; Mosaic Viruses - metabolism; Mosaic Viruses - pathogenicity; Mosaic Viruses - radiation effects; Nitrites - pharmacology; Plant Diseases; Plant Viruses - pathogenicity; Protein Binding; Radiation Effects; RNA, Viral - analysis; RNA, Viral - metabolism; RNA, Viral - pharmacology; RNA, Viral - radiation effects; Spectrophotometry, Ultraviolet; Ultraviolet Rays; Viral Proteins - metabolism
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-18-1-1

Department of Plant Pathology, University of Missouri, Columbia, Missouri 65201, U.S.A. Upon treatment with nitrous acid, southern bean mosaic virus (SBMV) was inactivated twice as fast as its isolated RNA. Nitrous acid treatment of SBMV resulted in appreciable binding of the virus protein to the nucleic acid. The extent of deamination of aminoribonucleotides, of intraparticle RNA and isolated SBMV-RNA by nitrous acid was identical. SBMV and SBMV-RNA were inactivated at the same rate following exposure to u.v. light. Furthermore, the extent of photoreactivation of the u.v.-irradiated SBMV and SBMV-RNA was similar. U.v. irradiation did not induce any detectable binding of virus protein to RNA in SBMV. Received 27 June 1972; accepted 21 August 1972.