infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA

John Innes Institute, Colney Lane, Norwich, NOR 70F, U.K. Tobacco protoplasts were infected with cowpea chlorotic mottle virus and its RNA in the presence of poly- L -ornithine to yield 10 6 to 10 7 particles per infected protoplast representing a probable increase in virus concentration of at least...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 1973-08, Vol.20 (2), p.177-193
Main Authors: Motoyoshi, F, Bancroft, J.B, Watts, J.W, Burgess, J
Format: Article
Language:English
Subjects:
Cytoplasm - microbiology
Fluorescent Antibody Technique
Hydrogen-Ion Concentration
Microscopy, Electron
Microscopy, Fluorescence
Mosaic Viruses - growth & development
Nicotiana
Ornithine - pharmacology
Plant Diseases
plant health
plant viruses
Plant Viruses - growth & development
Plant Viruses - immunology
Plant Viruses - pathogenicity
Plants, Toxic
Protoplasts - cytology
Protoplasts - microbiology
RNA, Viral - pharmacology
Stereoisomerism
Tobacco Mosaic Virus - growth & development
Virus Replication
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:John Innes Institute, Colney Lane, Norwich, NOR 70F, U.K. Tobacco protoplasts were infected with cowpea chlorotic mottle virus and its RNA in the presence of poly- L -ornithine to yield 10 6 to 10 7 particles per infected protoplast representing a probable increase in virus concentration of at least 1000-fold by 24 to 72 h after inoculation. The optimum inoculum input for virus was about 0.5 µg/ml and for RNA about 1.2 µg/ml; about 60 and 7%, respectively, of the protoplasts became infected, as judged by fluorescent antibody staining. Data relating to growth curves, inoculum and poly- L -ornithine concentrations, and pH effects are presented along with electron micrographs of sectioned protoplasts. Received 14 February 1973; accepted 12 March 1973.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-20-2-177