The Effects of Herpes Simplex Virus Type 1 on Cellular DNA-dependent RNA Polymerase Activities

Department of Biochemistry, Tennis Court Road, Cambridge, England An investigation of the activity of nuclear RNA polymerase following infection of LS cells with HSV-1 shows a decline in both major activities. This effect is not entirely due to inhibition of cellular protein synthesis, and the effec...

Full description

Saved in:
Bibliographic Details
Published in:Journal of general virology 1976-12, Vol.33 (3), p.471-482
Main Authors: Preston, C. M, Newton, Alison A
Format: Article
Language:English
Subjects:
Amanitins - pharmacology
Cell Line
Cell Nucleus - enzymology
Cycloheximide - pharmacology
Dactinomycin - pharmacology
DNA, Viral - metabolism
DNA-Directed RNA Polymerases - metabolism
Kinetics
Magnesium - pharmacology
Osmolar Concentration
Protein Biosynthesis
RNA - biosynthesis
RNA, Viral - biosynthesis
Simplexvirus - growth & development
Transcription, Genetic
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Department of Biochemistry, Tennis Court Road, Cambridge, England An investigation of the activity of nuclear RNA polymerase following infection of LS cells with HSV-1 shows a decline in both major activities. This effect is not entirely due to inhibition of cellular protein synthesis, and the effect of -amanitin-sensitive RNA polymerase is mediated by a protein(s) synthesized in the infected cell. Changes in the properties of this RNA polymerase activity include a reduction in the relative UTP/GTP incorporation ratio and an increased sensitivity to inhibition by actinomycin D, indicating that RNA polymerase II is involved in virus transcription. * Present address: Institute of Virology, Church Street, Glasgow G11 5JR, Scotland. Received 8 April 1976; accepted 14 July 1976.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-33-3-471