Translation of the Satellite RNA of Tomato Black Ring Virus in vitro and in Tobacco Protoplasts

Institut de Biologie Moléculaire et Cellulaire, 15 rue Descartes, 67000 Strasbourg, France * Scottish Horticultural Research Institute, Invergowrie, Dundee DD2 5DA, U.K. Translation of the satellite RNA (RNA-3) of tomato black ring virus (TBRV) in wheat germ extracts or reticulocyte lysates resulted...

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Bibliographic Details
Published in:Journal of general virology 1978-09, Vol.40 (3), p.587-593
Main Authors: Fritsch, Christiane, Mayo, M. A, Murant, A. F
Format: Article
Language:English
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Summary:Institut de Biologie Moléculaire et Cellulaire, 15 rue Descartes, 67000 Strasbourg, France * Scottish Horticultural Research Institute, Invergowrie, Dundee DD2 5DA, U.K. Translation of the satellite RNA (RNA-3) of tomato black ring virus (TBRV) in wheat germ extracts or reticulocyte lysates resulted in the synthesis of a polypeptide of mol. wt. about 48000, both in the presence and in the absence of RNA-1 and RNA-2. The RNA-3 specific polypeptide of TBRV strain G was slightly larger than that of strain S. A polypeptide of the same electrophoretic mobility as the in vitro translation product of RNA-3 was found in extracts of protoplasts infected with an isolate of TBRV-S possessing RNA-3 but not in extracts of protoplasts infected with an isolate lacking RNA-3. The lack of phenotypic effect of RNA-3 raises the question of the function of this protein in the infected plant. Received 3 March 1978; accepted 20 April 1978.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-40-3-587