Characterization and identification of corrosive bacterium isolated from petroleum product transporting pipelines

• Published in: Access microbiology 2019-03, Vol.1 (1A)
• Main Authors: Pedramfar, Arezoo, Maal, Keivan Beheshti, Mirdamadian, Sayed Hossein
• Format: Article
• Language: English
• ISSN: 2516-8290; 2516-8290
• DOI: 10.1099/acmi.ac2019.po0036

Corrosion is the main feature of the oil pipelines destruction. Biocorrosion has been detected in various industries, especially in the oil industry. The rusted pipes samples were obtained from the Gandomkar petroleum pipeline station, Iran. For screening the corrosion producing bacteria, the samples were cultured in the selective culture medium, manganese agar and Iron oxidizing agar incubated at 30 °C for 18 h. The purified individual colonies were subjected to macroscopic, microscopic and molecular examinations, respectively. The cultivation of corrosion based material on manganese agar isolated cream-coloured colonies, convex with the surrounding smooth. The microscopic examinations showed Gram-negative coccobacilli. Based on macroscopic, microscopic and molecular examinations the bacterial isolate was identified as Stenotrophomonas maltophilia strains PBM-IAUF-4 with the accession number of KU145280.1 in Gene Bank. The cultivation of corrosion based material on Iron oxidizing agar isolated cream-coloured colonies cream-coloured colonies that had swarming, convex. The results showed Gram-negative bacilli. Based on macroscopic, microscopic and molecular examinations the bacterial isolate was identified as Kluyvera intermedia strains PBM-IAUF-1 with the accession number of KU145277.1 in Gene Bank. This is the first report of isolation and identification of corrosion-producing bacteria from, Gandomkar, Iran. The first isolated bacterium was identified as Stenotrophomonas maltophilia . The second isolated bacterium was identified Kluyvera intermedia. Both bacteria were isolated for the first time in the world from pipeline corrosion samples. This study confirmed the role of bacteria in the corrosion of oil pipelines.