lys5 mutations of barley reveal the nature and importance of plastidial ADP-Glc transporters for starch synthesis in cereal endosperm

• Published in: Plant physiology (Bethesda) 2004-08, Vol.135 (4), p.2088-2097
• Main Authors: Patron, N.J, Greber, B, Fahy, B.F, Laurie, D.A, Parker, M.L, Denyer, K
• Format: Article
• Language: English
• Subjects: Adenosine Diphosphate Glucose - metabolism; ADP-glucose pyrophosphorylase; ADP-glucose transporter; alleles; Amino Acid Sequence; amino acid sequences; amino acid substitution; Barley; bioaccumulation; Biochemical Processes and Macromolecular Structures; Biological and medical sciences; biosynthesis; chromosome mapping; Corn; cytosol; Edible Grain - metabolism; Endosperm; Enzymes; Fundamental and applied biological sciences. Psychology; Genetic mutation; glucose transporters; grain crops; grains; Hordeum - genetics; Hordeum - metabolism; Hordeum vulgare; Lysine; Metabolism; Microscopy, Electron, Scanning; mitochondria; Models, Molecular; Molecular Sequence Data; Monosaccharide Transport Proteins - genetics; Monosaccharide Transport Proteins - metabolism; Mutagenesis; mutants; nucleotide-sugar transporter; nucleotidyltransferases; Organelles - genetics; Organelles - metabolism; Organelles - ultrastructure; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; plant proteins; Plants; Plastids; Plastids - genetics; Plastids - metabolism; Plastids - ultrastructure; Protein Structure, Secondary; Proteins; Sequence Alignment; sequence analysis; Sequence Homology, Amino Acid; Sequencing; starch; Starch - biosynthesis; starch granules; Starches; Uridine Diphosphate Glucose - metabolism
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.104.045203

Much of the ADP-Glc required for starch synthesis in the plastids of cereal endosperm is synthesized in the cytosol and transported across the plastid envelope. To provide information on the nature and role of the plastidial ADP-Glc transporter in barley (Hordeum vulgare), we screened a collection of low-starch mutants for lines with abnormally high levels of ADP-Glc in the developing endosperm. Three independent mutants were discovered, all of which carried mutations at the lys5 locus. Plastids isolated from the lys5 mutants were able to synthesize starch at normal rates from Glc-1-P but not from ADP-Glc, suggesting a specific lesion in the transport of ADP-Glc across the plastid envelope. The major plastidial envelope protein was purified, and its sequence showed it to be homologous to the maize (Zea mays) ADP-Glc transporter BRITTLE1. The gene encoding this protein in barley, Hv.Nst1, was cloned, sequenced, and mapped. Like lys5, Hv.Nst1 lies on chromosome 6(6H), and all three of the lys5 alleles that were examined were shown to carry lesions in Hv.Nst1. Two of the identified mutations in Hv.Nst1 lead to amino acid substitutions in a domain that is conserved in all members of the family of carrier proteins to which Hv.NST1 belongs. This strongly suggests that Hv.Nst1 lies at the Lys5 locus and encodes a plastidial ADP-Glc transporter. The low-starch phenotype of the lys5 mutants shows that the ADP-Glc transporter is required for normal rates of starch synthesis. This work on Hv.NST1, together with the earlier work on BRITTLE1, suggests that homologous transporters are probably present in the endosperm of all cereals.