Macromolecular Cryocrystallography

The current techniques for X‐ray‐diffraction data collection from macromolecular crystals at cryogenic temperatures are reviewed. The development of the experimental methods is outlined and the basic concepts pertaining to radiation damage and cryoprotection are summarized. Emphasis is placed on the...

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Bibliographic Details
Published in:Journal of applied crystallography 1997-06, Vol.30 (3), p.211-237
Main Authors: Garman, E. F., Schneider, T. R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Condensed matter: structure, mechanical and thermal properties
Crystalline structure
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Molecular biophysics
Physics
Single-crystal and powder diffraction
Structure in molecular biology
Structure of solids and liquids
crystallography
X-ray diffraction and scattering
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Summary:The current techniques for X‐ray‐diffraction data collection from macromolecular crystals at cryogenic temperatures are reviewed. The development of the experimental methods is outlined and the basic concepts pertaining to radiation damage and cryoprotection are summarized. Emphasis is placed on the practical aspects important to the success of the techniques, and a detailed account of these is presented.
ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S0021889897002677