Crystallization and preliminary X-ray diffraction studies of cytochrome c 6 from Porphyra yezoensis

Cytochrome c 6 from the red alga Porphyra yezoensis has been purified and crystallized by the sitting-drop vapour-diffusion method. Two different crystal forms, tetragonal and orthorhombic, were obtained. The tetragonal crystals belong to space group P 4 1 2 1 2 or P 4 3 2 1 2, with unit-cell dimens...

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Bibliographic Details
Published in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2000-01, Vol.56 (1), p.79-80
Main Authors: Sasaki, Tomokazu, Nakahara, Megumi, Matsuda, Aya, Yamasaki, Takenobu, Katoh, Sakae, Ohshima, Shigeru, Oonishi, Isao, Uchida, Akira
Format: Article
Language:English
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Summary:Cytochrome c 6 from the red alga Porphyra yezoensis has been purified and crystallized by the sitting-drop vapour-diffusion method. Two different crystal forms, tetragonal and orthorhombic, were obtained. The tetragonal crystals belong to space group P 4 1 2 1 2 or P 4 3 2 1 2, with unit-cell dimensions a = 49.33 (2), c = 83.70 (10) Å. The orthorhombic crystals belong to space group P 2 1 2 1 2 1 , with unit-cell dimensions a = 46.74 (2), b = 49.42 (1), c = 37.11 (1) Å. Absorption spectra of the crystals showed that the tetragonal form was oxidized and the orthorhombic form was reduced.
ISSN:0907-4449
DOI:10.1107/S0907444999013888