A synchrotron radiation X-ray scattering study of aqueous solutions of native DNA

Synchrotron radiation small‐angle X‐ray scattering (SAXS) was used to investigate solutions of native DNA at different ionic strengths and temperatures. The mass per unit length, radius of gyration of the cross‐section of DNA and apparent second virial coefficient (A2) were obtained from Zimm plots...

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Bibliographic Details
Published in:Journal of synchrotron radiation 1999-09, Vol.6 (5), p.1031-1034
Main Authors: Barone, Giampaolo, Sayers, Zehra, Svergun, Dmitri, Koch, Michel H. J.
Format: Article
Language:English
Subjects:
DNA
short-range order
thermal denaturation
X-ray solution scattering
Zimm plot
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Summary:Synchrotron radiation small‐angle X‐ray scattering (SAXS) was used to investigate solutions of native DNA at different ionic strengths and temperatures. The mass per unit length, radius of gyration of the cross‐section of DNA and apparent second virial coefficient (A2) were obtained from Zimm plots in the rodlike particle approximation. The values of A2 obtained in this way are positive and almost constant indicating that the repulsive interactions still influence the scattering patterns at resolutions as high as 5–8 nm. SAXS measurements in continuous temperature scans indicate that the rod approximation is valid over a wide temperature range during DNA melting and confirm that the rodlike–wormlike transition temperature increases with ionic strength.
ISSN:1600-5775
0909-0495
1600-5775
DOI:10.1107/S0909049599007463