Redefining the hepatoprotective potential of Javanese turmeric (Curcuma xanthorrhiza) Kombucha towards the diethylnitrosamine-induced hepatotoxicity of mice

• Published in: Nutrition and food science 2024-04, Vol.54 (3), p.483-494
• Main Authors: Zubaidah, Elok, Dea, Eirene Charista, Saparianti, Ella, Putri, Rhytia Ayu Christianty, Sujuti, Hidayat, Srianta, Ignatius, Godelive, Laura, Tewfik, Ihab
• Format: Article
• Language: English
• Subjects: Appetite; Beverages; Bioavailability; Biomarkers; Black tea; Blood; Blood levels; Diet; Diethylnitrosamine; Enzymes; Exercise; Fermentation; Fermented food; Free radicals; Hepatocytes; Hepatotoxicity; Herbal medicine; Histology; Inflammation; ISO standards; Liver; Liver diseases; Malondialdehyde; Musculoskeletal system; Oxidative stress; Pyruvic acid; Rodents; Substrates; Tea; Transaminase; Transaminases; Variance analysis
• ISSN: 0034-6659; 1758-6917; 0034-6659
• DOI: 10.1108/NFS-09-2023-0219

Purpose This research intended the utilization of Javanese turmeric (0.4% w/v) as a kombucha substrate and analysis of its hepatoprotective activity, in comparison against nonfermented Javanese turmeric beverage (JTB) and black tea kombucha. Design/methodology/approach Forty-two healthy male Balb/c mice (two- to three-week-old, 20–30 g) were divided into six groups with seven replicates each. The treatments were normal diet, normal diet + Javanese turmeric kombucha (JTK), normal diet + diethylnitrosamine (DEN), DEN + JTB, DEN + JTK, DEN + black tea kombucha. Kombuchas and JTB were given at 0.3 mL/20 g BW/d. DEN was induced intraperitoneally at a dose of 100 mg/kg. Observed biomarkers were blood serum glutamate pyruvate transaminase (SGPT) and serum glutamate oxaloacetate transaminase (SGOT) activity, serum malonaldehyde (MDA), as well as liver histology. Data were analyzed using analysis of variance. Findings Among DEN-induced groups, JTK significantly (p < 0.05) diminished the level of blood SGPT, SGOT and serum MDA. JTK also had lower blood SGPT (8.604 ± 2.195 U/L) and serum MDA levels (2.884 ± 0.083 nM/mL) compared to the normal group (8.604 ± 2.195 U/L and 5.050 ± 0.998 nM/mL, respectively). JTK also produced the least damaged liver-cell numbers. Originality/value JTK demonstrated better hepatoprotective activity compared to JTB.