A Multimodal CMOS MEA for High-Throughput Intracellular Action Potential Measurements and Impedance Spectroscopy in Drug-Screening Applications

Multi-electrode arrays (MEAs) are a candidate technology to screen cardiotoxicity in vitro because they enable noninvasive recording of cardiac beating rate, electrical field potential duration, and other parameters. In this paper, we present an active MEA chip featuring 16 384 electrodes, 1024 simu...

Full description

Saved in:
Bibliographic Details
Published in:IEEE journal of solid-state circuits 2018-11, Vol.53 (11), p.3076-3086
Main Authors: Lopez, Carolina Mora, Chun, Ho Sung, Wang, Shiwei, Berti, Laurent, Putzeys, Jan, Van Den Bulcke, Carl, Weijers, Jan-Willem, Firrincieli, Andrea, Reumers, Veerle, Braeken, Dries, Van Helleputte, Nick
Format: Article
Language:English
Subjects:
Active electrode
Biocompatibility
cardiotoxicity
Channels
CMOS
Computer architecture
drug screening
Drugs
electrical recording
electrical stimulation
Electrodes
Extracellular
high throughput
Impedance
Impedance spectroscopy
impedance spectroscopy (IS)
intracellular recording
Microprocessors
Monitoring
multi-electrode array (MEA)
multi-modality
Power consumption
Recording
Screening
Silicon substrates
Spectrum analysis
Stimulation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Multi-electrode arrays (MEAs) are a candidate technology to screen cardiotoxicity in vitro because they enable noninvasive recording of cardiac beating rate, electrical field potential duration, and other parameters. In this paper, we present an active MEA chip featuring 16 384 electrodes, 1024 simultaneous readout channels, and 64 stimulation units (SUs) to enable six different cell-interfacing modalities: extracellular and intracellular recording, current and voltage stimulation, fast impedance motoring, and impedance spectroscopy (IS). The chip was designed and fabricated in a 0.13- \mu \text{m} Al CMOS technology, and the electrodes were fabricated on top of the silicon substrate using biocompatible TiN. Measurement results show a total input-referred noise of 7.5 \pm 0.6~\mu \text{V}_{\mathrm{rms}} and 12.0 \pm 2.4~\mu \text{V}_{\mathrm {rms}} for the action potential and full bands, respectively, with a total power consumption of 95 mW for 1024 readout channels. We have experimentally validated the CMOS MEA in in vitro experiments, demonstrating all the different modalities. This novel platform will potentially enable high-throughput electrical activity monitoring and drug screening of cardiomyocytes.
ISSN:0018-9200
1558-173X
DOI:10.1109/JSSC.2018.2863952