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Mechanisms of Epi-Detected Stimulated Raman Scattering Microscopy
Epi-detection is indispensible for stimulated Raman scattering (SRS) imaging of opaque samples. We present an analysis of the mechanisms underlying the generation of epi-detected SRS signals. By study of forward- and epi-detected SRS signals from pharmaceutical films of controlled thickness and scat...
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Published in: | IEEE journal of selected topics in quantum electronics 2012-01, Vol.18 (1), p.384-388 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Epi-detection is indispensible for stimulated Raman scattering (SRS) imaging of opaque samples. We present an analysis of the mechanisms underlying the generation of epi-detected SRS signals. By study of forward- and epi-detected SRS signals from pharmaceutical films of controlled thickness and scatterer density, we show that the epi-detected SRS signal arises from the backscattering of the forward-propagating probe photons. Furthermore, we show that both forward- and epi-detected SRS signal intensity linearly depends on the local oscillator power at the detector at the same slope. Under the same excitation conditions, stimulated Raman loss produces a larger epi-detected signal than stimulated Raman gain due to the larger scattering cross section of light with a shorter wavelength. |
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ISSN: | 1077-260X 1558-4542 |
DOI: | 10.1109/JSTQE.2011.2157463 |