Development of a TLR1/TLR2-Based Chemiresistive Biosensor for Ultra-Sensitive Gram-Positive Bacterial Detection Using Amine-Terminated Carbon Surfaces

• Published in: IEEE sensors letters 2024-12, Vol.8 (12), p.1-4
• Main Authors: Gangwar, Rahul, Supraja, Patta, Rao, Karri Trinadha, Tripathy, Suryasnata, Singh, Shiv Govind, Vanjari, Siva Rama Krishna
• Format: Article
• Language: English
• Subjects: Accuracy; amine-terminated carbon surface; Bacteria; Biosensors; Carbon; Chemical and biological sensors; Chemical sensors; chemiresistive biosensor; gram-positive bacteria; Real-time systems; Sensitivity; Sensors; Surface treatment; toll-like receptors (TLRs); Wounds
• ISSN: 2475-1472; 2475-1472
• DOI: 10.1109/LSENS.2024.3496995

Accurate detection of gram-positive bacterial colonies is essential for managing chronic wounds and overcoming delays in healing, as these bacteria can worsen wound conditions and impede recovery. This study introduces a cost-effective electrochemical sensing platform designed to support healthcare professionals in making timely, targeted treatment decisions. We developed the platform using chemically functionalized amine-terminated carbon surfaces combined with the TLR1/TLR2 heterodimer complex to detect gram-positive bacteria. The biosensors featuring these advanced carbon surfaces demonstrated superior performance due to their high surface area and efficient electron transfer capabilities. The TLR1/TLR2-based sensors accurately identified gram-positive bacteria, with a theoretical detection limit of 0.0413 CFU/mL. The sensors also exhibited high selectivity and sensitivity, with a response rate of 220.878 ((ΔR/R)/CFU/mL)/cm 2 for the amine-terminated carbon surfaces. This novel electrochemical sensing platform provides an effective solution for real-time detection and management of gram-positive bacterial infections in chronic wound care.