The Role of Pulse Repetition Rate in nsPEF-Induced Electroporation: A Biological and Numerical Investigation

The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells were analyzed...

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Bibliographic Details
Published in:IEEE transactions on biomedical engineering 2015-09, Vol.62 (9), p.2234-2243
Main Authors: Lamberti, Patrizia, Romeo, Stefania, Sannino, Anna, Zeni, Luigi, Zeni, Olga
Format: Article
Language:English
Subjects:
biological effects
Biology
Biomembranes
Cell Membrane - radiation effects
Cell Survival - radiation effects
Electric fields
electroporation
Electroporation - methods
Fluorescence
Histograms
Humans
Jurkat Cells
Models, Theoretical
nanosecond pulsed electric field
Nanotechnology
numerical analysis
Numerical models
Plasmas
Porosity - radiation effects
pulse repetition rate
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Summary:The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells were analyzed after exposure to 500, 1.3 MV/m, 40 ns PEFs with variable PRR (2-30 Hz). A finite-element model was used to investigate EP dynamics in a single cell under the same pulsing conditions, by looking at the time course of transmembrane voltage and pore density on the ns time scale. The biological observations showed an increased EP and reduced viability of the exposed cells at lower PRR in the considered range. The numerical analysis resulted in different dynamics of plasma membrane response when ns pulses were delivered with different PRR, consistently with a phenomenon of electrodesensitization recently hypothesized by another research group.
ISSN:0018-9294
1558-2531
DOI:10.1109/TBME.2015.2419813