The Role of Pulse Repetition Rate in nsPEF-Induced Electroporation: A Biological and Numerical Investigation

• Published in: IEEE transactions on biomedical engineering 2015-09, Vol.62 (9), p.2234-2243
• Main Authors: Lamberti, Patrizia, Romeo, Stefania, Sannino, Anna, Zeni, Luigi, Zeni, Olga
• Format: Article
• Language: English
• Subjects: biological effects; Biology; Biomembranes; Cell Membrane - radiation effects; Cell Survival - radiation effects; Electric fields; electroporation; Electroporation - methods; Fluorescence; Histograms; Humans; Jurkat Cells; Models, Theoretical; nanosecond pulsed electric field; Nanotechnology; numerical analysis; Numerical models; Plasmas; Porosity - radiation effects; pulse repetition rate
• ISSN: 0018-9294; 1558-2531
• DOI: 10.1109/TBME.2015.2419813

The impact of pulse repetition rate (PRR) in modulating electroporation (EP) induced by nanosecond pulsed electric fields (nsPEFs) in mammalian cells was approached here by performing both biological and numerical analysis. Plasma membrane permeabilization and viability of Jurkat cells were analyzed after exposure to 500, 1.3 MV/m, 40 ns PEFs with variable PRR (2-30 Hz). A finite-element model was used to investigate EP dynamics in a single cell under the same pulsing conditions, by looking at the time course of transmembrane voltage and pore density on the ns time scale. The biological observations showed an increased EP and reduced viability of the exposed cells at lower PRR in the considered range. The numerical analysis resulted in different dynamics of plasma membrane response when ns pulses were delivered with different PRR, consistently with a phenomenon of electrodesensitization recently hypothesized by another research group.