Simplified Fabrication of Magnetic Nanoparticles With Directly Adsorbed Antibodies for Bacteria Detection

Antigen-antibody reactions with conjugated magnetic nanoparticles (MNPs) are widely used for detecting viruses, bacteria, and proteins. In this study, we proposed a novel method for adsorbing antibodies directly on the surface of MNPs without coating any proteins. MNPs (Fe 3 O 4 ; average size 4 nm)...

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Bibliographic Details
Published in:IEEE transactions on magnetics 2022-08, Vol.58 (8), p.1-6
Main Authors: Yoneyama, Tsuyoshi, Kuwahata, Akihiro, Murayama, Toru, Tonthat, Loi, Yabukami, Shin, Sato, Yuya, Teramura, Yuji, Ikeda-Ohtsubo, Wakako, Ogawa, Tomoyuki
Format: Article
Language:English
Subjects:
Antibodies
Antibody conjugation
Antigens
antigen–antibody reaction
Bacteria
bacteria detection
Iron
Iron oxides
Magnetic moments
magnetic nanoparticle (MNP)
Magnetic nanoparticles
Magnetic resonance imaging
Magnetism
Microscopy
Nanoparticles
Proteins
Saturation magnetization
Thermal decomposition
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Summary:Antigen-antibody reactions with conjugated magnetic nanoparticles (MNPs) are widely used for detecting viruses, bacteria, and proteins. In this study, we proposed a novel method for adsorbing antibodies directly on the surface of MNPs without coating any proteins. MNPs (Fe 3 O 4 ; average size 4 nm) were synthesized by a thermal decomposition method using oleyl amine as a surfactant, the fabricated MNPs exhibit superparamagnetic characteristics, and the antibodies (anti- Bifidobacterium longum ) were adsorbed. We found that 15 \mu \text{g} /mg of antibody adsorbed to MNPs and were able to detect the bacteria bound to the produced antibody-MNPs based on the antigen-antibody reaction, offering potential for highly sensitive bacteria detection.
ISSN:0018-9464
1941-0069
DOI:10.1109/TMAG.2022.3168360