Decreased expression of ectonucleotidase E‐ NPP 1 in leukocytes from subjects with severe asthma exacerbation

• Published in: Allergy (Copenhagen) 2016-01, Vol.71 (1), p.124-128
• Main Authors: Montaño, L. M., Vargas, M. H., Díaz‐Hernández, V., De Ita, M., Kazakova, R., Barajas‐López, C.
• Format: Article
• Language: English
• ISSN: 0105-4538; 1398-9995
• DOI: 10.1111/all.12772

Several studies suggest that ATP and related nucleotides play a role in the pathophysiology of asthma. However, the functionality of ectonucleotidases in this disease has been scantly investigated. We studied total ectonucleotidase activity in leukocytes from patients suffering from asthma exacerbation and explored the expression of E‐ NTPD ase 1, 2, 3, and 8, and E‐ NPP 1, 2, and 3, in their polymorphonuclear cells by immunofluorescence and qPCR . Leukocytes from patients with mild or moderate asthma exacerbation had similar ectonucleotidase activity than leukocytes from healthy subjects, while in patients with severe asthma exacerbation, this activity was lower. Of the ectonucleotidases studied, only E‐ NPP 1 displayed diminished immunofluorescence and a significant decrease in its mRNA expression, both in patients with severe asthma exacerbation. This reduced E‐ NPP 1 expression could be responsible for increased amounts of ATP or other nucleotides, capable of worsening asthma exacerbation, and warranting further investigation.