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Structure-guided Discovery of a Novel Non-peptide Inhibitor of Dengue Virus NS2B-NS3 Protease

Dengue fever is a fast emerging epidemic‐prone viral disease caused by dengue virus serotypes 1‐4. NS2B–NS3 protease of dengue virus is a validated target to develop antiviral agents. A major limitation in developing dengue virus protease inhibitors has been the lack of or poor cellular activity. In...

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Published in:Chemical biology & drug design 2015-09, Vol.86 (3), p.255-264
Main Authors: Li, Linfeng, Basavannacharya, Chandrakala, Chan, Kitti Wing Ki, Shang, Luqing, Vasudevan, Subhash G., Yin, Zheng
Format: Article
Language:English
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Summary:Dengue fever is a fast emerging epidemic‐prone viral disease caused by dengue virus serotypes 1‐4. NS2B–NS3 protease of dengue virus is a validated target to develop antiviral agents. A major limitation in developing dengue virus protease inhibitors has been the lack of or poor cellular activity. In this work, we extracted and refined a pharmacophore model based on X‐ray crystal structure and predicted binding patterns, followed by a three‐dimensional flexible database filtration. These output molecules were screened according to a docking‐based protocol, leading to the discovery of a compound with novel scaffold and good cell‐based bioactivity that has potential to be further optimized. The discovery of this novel scaffold by combination of in silico methods suggests that structure‐guided drug discovery can lead to the development of potent dengue virus protease inhibitors. Based on structural information of DENV NS2B–NS3 protease and previously reported lead compounds, we carried out a hierarchical virtual screening and biological activity measurement. Finally, we obtained several novel compounds that inhibited the protease, and one hit further demonstrated antiviral activity in cell‐based assay with the EC50 of 5 μm.
ISSN:1747-0277
1747-0285
DOI:10.1111/cbdd.12500