Bursaphelenchus fungivorus from P inus pinaster bark in P ortugal

Bursaphelenchus fungivorus is reported for the first time in P ortugal, identified as associated with P inus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphi...

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Bibliographic Details
Published in:Forest pathology = Journal de pathologie forestière = Zeitschrift für Forstpathologie 2014-04, Vol.44 (2), p.131-136
Main Authors: Fonseca, L., Cardoso, J. M. S., Moron‐Lopez, J., Abrantes, I.
Format: Article
Language:English
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Summary:Bursaphelenchus fungivorus is reported for the first time in P ortugal, identified as associated with P inus pinaster bark and characterized on the basis of morphological and morphometrical characters for this species. Species identification was confirmed using restriction fragment length polymorphism analysis and sequencing of the internal transcribed spacer ( ITS ) regions of ribosomal DNA . Intraisolate genetic variability was detected among ITS sequences of the P ortuguese B . fungivorus isolate. Phylogenetic analysis, obtained from multiple sequence alignment between ITS sequences of B ursaphelenchus species, revealed that the P ortuguese B . fungivorus isolate clusters with other B . fungivorus isolates, forming a separate group close to B . seani highlighting a molecular proximity of these two species.
ISSN:1437-4781
1439-0329
DOI:10.1111/efp.12077