Axonal growth towards X enopus skin in vitro is mediated by matrix metalloproteinase activity

We have previously demonstrated that the growth of peripheral nervous system axons is strongly attracted towards limb buds and skin explants in vitro . Here, we show that directed axonal growth towards skin explants of X enopus laevis in matrigel is associated with expression of matrix metalloprotei...

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Published in:The European journal of neuroscience 2013-02, Vol.37 (4), p.519-531
Main Authors: Tonge, David, Zhu, Ning, Lynham, Steven, Leclere, Pascal, Snape, Alison, Brewer, Alison, Schlomann, Uwe, Ferdous, Taheera, Tennyson, Charlene, Bartsch, Jörg W., Ward, Malcolm, Pizzey, John
Format: Article
Language:English
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Summary:We have previously demonstrated that the growth of peripheral nervous system axons is strongly attracted towards limb buds and skin explants in vitro . Here, we show that directed axonal growth towards skin explants of X enopus laevis in matrigel is associated with expression of matrix metalloproteinase ( MMP )‐18 and also other MMP s, and that this long‐range neurotropic activity is inhibited by the broad‐spectrum MMP inhibitors BB ‐94 and GM 6001. We also show that forced expression of MMP ‐18 in COS ‐7 cell aggregates enhances axonal growth from X enopus dorsal root ganglia explants. Nidogen is the target of MMP s released by cultured skin in matrigel, whereas other components remain intact. Our results suggest a novel link between MMP activity and extracellular matrix breakdown in the control of axonal growth.
ISSN:0953-816X
1460-9568
DOI:10.1111/ejn.12075