Expression of sarcoplasmic‐endoplasmic reticulum Ca‐ ATP ase isoforms in masticatory muscles

The aim of this study was to characterize the sarcoplasmic‐endoplasmic reticulum Ca‐ ATP ase ( SERCA ) isoforms in rabbit masticatory muscles compared with those in fast‐twitch muscle. It was hypothesized that combined expression of the SERCA isoforms in fast‐ and slow‐twitch muscles accounts for lo...

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Bibliographic Details
Published in:European journal of oral sciences 2014-02, Vol.122 (1), p.36-41
Main Authors: Sánchez, Gabriel A., Trinks, Pablo W., Richard, Susana B., Di Croce, Daniel E., Takara, Delia
Format: Article
Language:English
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Summary:The aim of this study was to characterize the sarcoplasmic‐endoplasmic reticulum Ca‐ ATP ase ( SERCA ) isoforms in rabbit masticatory muscles compared with those in fast‐twitch muscle. It was hypothesized that combined expression of the SERCA isoforms in fast‐ and slow‐twitch muscles accounts for lower Ca‐ ATP ase activity. SERCA was isolated by differential centrifugation, the isoforms were determined by ELISA , and the activity of each isoform was measured using a colorimetric method. Activity was tested for significance by anova , and the distribution of isoforms was assessed using the chi‐square test ( P  
ISSN:0909-8836
1600-0722
DOI:10.1111/eos.12098