Discrepant diagnostic results using two genotyping methods in a chronic hepatitis C patient in serogroup 1

We present the case of a chronic hepatitis C (CHC) patient who was originally diagnosed with genotype 2a on serogroup 1 CHC genotype testing, but who was subsequently confirmed to have genotype 1b when using the hepatitis C virus (HCV) monitor genotype assay. The genotype 2a diagnosis was attributed...

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Bibliographic Details
Published in:Hepatology research 2016-04, Vol.46 (4), p.354-356
Main Authors: Ishikawa, Toru, Abe, Satoshi, Kojima, Yuichi, Kojima, Shigeyuki, Yoshida, Toshiaki
Format: Article
Language:English
Subjects:
genotype
genotyping assay
hepatitis C virus
serotype
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Summary:We present the case of a chronic hepatitis C (CHC) patient who was originally diagnosed with genotype 2a on serogroup 1 CHC genotype testing, but who was subsequently confirmed to have genotype 1b when using the hepatitis C virus (HCV) monitor genotype assay. The genotype 2a diagnosis was attributed to the fact that the type 2a‐specific primer used in Okamoto et al.’s method (HCV genotype primer kit) has relatively high homology, which caused the amplification reaction to proceed, rendering a HCV RNA genotype test result of 2a. Genotype testing is important in determining whether a patient is indicated for concomitant dual oral therapy; however, the potential for different diagnoses such as described in this report highlights the importance of serogroup confirmation.
ISSN:1386-6346
1872-034X
DOI:10.1111/hepr.12536