IL-17A[G197G]-Association between NO x and gestational age in a South African birth cohort

• Published in: International journal of immunogenetics 2018-04, Vol.45 (2), p.54-62
• Main Authors: Nansook, P, Naidoo, R N, Muttoo, S, Asharam, K, Ramkaran, P, Phulukdaree, A, Chuturgoon, A A
• Format: Article
• Language: English
• ISSN: 1744-3121; 1744-313X
• DOI: 10.1111/iji.12358

Interleukin (IL-)17A, plays a role in pathogenic defence, but is implicated in chronic inflammatory diseases, and has recently been associated with variable pregnancy outcomes. We investigated the role of maternal IL-17-[G197A]-specific effects of third-trimester IL-17 mRNA expression, NO exposure levels and other variables on gestational age, in the Mother and Child in the Environment (MACE) birth cohort in South Africa. A total of 327 participants were genotyped for IL-17-[G197A] by polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP). Quantitative real-time PCR was used to quantitate IL-17-mRNA expression in whole blood. Multivariate linear regression analysis, stratified by IL-17-[G197A] genotype, was used to test for effects of NO , IL17A/GAPDH, haemoglobin, body mass index, HIV-1 positivity, maternal education and income level on gestational age. Lower expression was associated with the IL-17-GG versus GA in the cohort and HIV-1-negative group (p = .0007, p = .0058), while no difference was observed in the HIV-1 positives. Elevated IL-17A expression was observed in the high NO exposure groups, within IL-17[G197G] (p = .0004). IL-17[G197G] was associated with PTB (p