D e novo characterization of the D ialeurodes citri transcriptome: mining genes involved in stress resistance and simple sequence repeats ( SSRs) discovery

• Published in: Insect molecular biology 2014-02, Vol.23 (1), p.52-66
• Main Authors: Chen, E‐H., Wei, D‐D., Shen, G‐M., Yuan, G‐R., Bai, P‐P., Wang, J‐J.
• Format: Article
• Language: English
• ISSN: 0962-1075; 1365-2583
• DOI: 10.1111/imb.12060

The citrus whitefly, D ialeurodes citri ( A shmead), is one of the three economically important whitefly species that infest citrus plants around the world; however, limited genetic research has been focused on D . citri , partly because of lack of genomic resources. In this study, we performed de novo assembly of a transcriptome using I llumina paired‐end sequencing technology (Illumina Inc., San Diego, CA, USA). In total, 36 766 unigenes with a mean length of 497 bp were identified. Of these unigenes, we identified 17 788 matched known proteins in the National Center for Biotechnology Information database, as determined by B last search, with 5731, 4850 and 14 441 unigenes assigned to clusters of orthologous groups ( COG ), gene ontology ( GO ), and S wiss P rot, respectively. In total, 7507 unigenes were assigned to 308 known pathways. In‐depth analysis of the data showed that 117 unigenes were identified as potentially involved in the detoxification of xenobiotics and 67 heat shock protein ( Hsp ) genes were associated with environmental stress. In addition, these enzymes were searched against the GO and COG database, and the results showed that the three major detoxification enzymes and Hsps were classified into 18 and 3, 6, and 8 annotations, respectively. In addition, 149 simple sequence repeats were detected. The results facilitate the investigation of molecular resistance mechanisms to insecticides and environmental stress, and contribute to molecular marker development. The findings greatly improve our genetic understanding of D . citri , and lay the foundation for future functional genomics studies on this species.