Calcium-mediated DAD in membrane potentials and triggered activity in atrial myocytes of ETV1 f / f MyHC Cre /+ mice

The E-twenty-six variant 1 (ETV1)-dependent transcriptome plays an important role in atrial electrical and structural remodelling and the occurrence of atrial fibrillation (AF), but the underlying mechanism of ETV1 in AF is unclear. In this study, cardiomyocyte-specific ETV1 knockout (ETV1 MyHC , ET...

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Published in:Journal of cellular and molecular medicine 2024-08, Vol.28 (16), p.e70005
Main Authors: Fang, Li-Hua, Chen, Qian, Cheng, Xian-Lu, Li, Xiao-Qian, Zou, Tian, Chen, Jian-Quan, Xiang, Guo-Jian, Xue, Qiao, Li, Yang, Zhang, Jian-Cheng
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Atrial Fibrillation - genetics
Atrial Fibrillation - metabolism
Calcium - metabolism
Calcium Signaling
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Heart Atria - metabolism
Male
Membrane Potentials
Mice
Mice, Knockout
Myocytes, Cardiac - metabolism
Transcription Factors - genetics
Transcription Factors - metabolism
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Summary:The E-twenty-six variant 1 (ETV1)-dependent transcriptome plays an important role in atrial electrical and structural remodelling and the occurrence of atrial fibrillation (AF), but the underlying mechanism of ETV1 in AF is unclear. In this study, cardiomyocyte-specific ETV1 knockout (ETV1 MyHC , ETV1-CKO) mice were constructed to observe the susceptibility to AF and the underlying mechanism in AF associated with ETV1-CKO mice. AF susceptibility was examined by intraesophageal burst pacing, induction of AF was increased obviously in ETV1-CKO mice than WT mice. Electrophysiology experiments indicated shortened APD and APD , increased incidence of DADs, decreased density of I in ETV1-CKO mice. There was no difference in V and V , but a significantly longer duration of the recovery time after inactivation in the ETV1-CKO mice. The recording of intracellular Ca showed that there was significantly increased in the frequency of calcium spark, Ca transient amplitude, and proportion of SCaEs in ETV1-CKO mice. Reduction of Cav1.2 rather than NCX1 and SERCA2a, increase RyR2, p-RyR2 and CaMKII was reflected in ETV1-CKO group. This study demonstrates that the increase in calcium spark and SCaEs corresponding to Ca transient amplitude may trigger DAD in membrane potential in ETV1-CKO mice, thereby increasing the risk of AF.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.70005