First Molecular Characterization of C ryptosporidium spp. Infecting Buffalo Calves in Brazil

With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was po...

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Bibliographic Details
Published in:The Journal of eukaryotic microbiology 2015-09, Vol.62 (5), p.657-661
Main Authors: Aquino, Monally C. C., Widmer, Giovanni, Zucatto, Anaiza S., Viol, Milena A., Inácio, Sandra V., Nakamura, Alex A., Coelho, Willian M. D., Perri, Silvia H. V., Meireles, Marcelo V., Bresciani, Katia D. S.
Format: Article
Language:English
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Summary:With the aim of determining the occurrence of Cryptosporidium spp., 222 fecal samples were collected from Murrah buffalo calves aged up to 6 mo. Fecal DNA was genotyped with a nested polymerase chain reaction targeting the 18S rRNA gene and sequencing of the amplified fragment. Nested 18S PCR was positive for 48.2% of the samples. Sequence analysis showed that the most frequent species in these animals was Cryptosporidium ryanae , which was present in buffalo calves as young as 5 d. The zoonotic species Cryptosporidium parvum was detected in one animal. An uncommon Cryptosporidium 18S genotype was found in buffaloes.
ISSN:1066-5234
1550-7408
DOI:10.1111/jeu.12223