Mulberry (Morus atropurpurea Roxb.) leaf polyphenols inhibits adipogenesis and lipogenesis‐related gene expression in 3T3‐L1 adipocytes

• Published in: Journal of food biochemistry 2018-10, Vol.42 (5), p.e12599-n/a
• Main Authors: Li, Qian, Dai, Yanli, Zou, Yuxiao, Liao, Sentai, Shen, Weizhi, Hu, Tenggen, Liu, Fan
• Format: Article
• Language: English
• Subjects: 3T3‐L1 adipocytes; antiobesity; lipid accumulation; Morus atropurpurea Roxb; Mulberry leaf polyphenols
• ISSN: 0145-8884; 1745-4514
• DOI: 10.1111/jfbc.12599

The effects of mulberry leaf polyphenols (MLPs) on the proliferation and differentiation of 3T3‐L1 preadipocytes were investigated in this study. No significant inhibitory effect on cell proliferation when MLP content was 10–50 μg/mL. MLP inhibited lipid accumulation in 3T3‐L1 preadipocytes dose‐dependently at concentrations of 0–50 μg/mL. The treatment of differentiating 3T3‐L1 cells with MLP at concentration of 40–50 μg/mL significantly inhibited the spillage of free fatty acids (FFAs), reduced the intracellular total triglyceride (TG) and cholesterol (TC) concentration. Furthermore, a decrease in mRNA expression of peroxisome proliferator activated receptor‐γ and CCAAT‐enhancer‐binding protein‐α, as well as fatty acid synthase and adiponectin were observed. This is the first study to demonstrate that MLP attenuated adipogenesis‐related mRNA expression of 3T3‐L1 preadipocytes. The findings suggested that MLP has complimentary potency for the regulation of obesity. Practical application The effects of MLP on the proliferation and differentiation of 3T3‐L1 preadipocytes were investigated for the first time in this study. MLP decreased FAA, TC, TG, lipid accumulation and attenuated adipogenesis‐related mRNA expression in differentiating 3T3‐L1 cells. The result clearly identified mulberry leaf supplements could be used as a nutraceutical agent to ameliorate obesity and its complications