Detoxification of Aflatoxin B 1 and M 1 by Lactobacillus acidophilus and prebiotics in whole cow's milk

• Published in: Journal of food safety 2019-10, Vol.39 (5)
• Main Authors: Wochner, Katia F., Moreira, Maria C. C., Kalschne, Daneysa L., Colla, Eliane, Drunkler, Deisy A.
• Format: Article
• Language: English
• ISSN: 0149-6085; 1745-4565
• DOI: 10.1111/jfs.12670

The detoxification ability of Lactobacillus acidophilus and prebiotics was evaluated by a Plackett‐Burman Design to examine the reduction of concentration and bioaccessibility of aflatoxin B 1 (AFB 1 ) and M 1 (AFM 1 ) in artificially contaminated whole cow's milk. Six variables were evaluated: AFB 1 (3.25–6.0 μg L −1 ) or AFM 1 (1.0–2.0 μg L −1 ) concentration; incubation time (0–6 h); and inulin, oligofructose, β‐glucan, and polydextrose concentrations (each between 0.00 and 0.75%). All runs achieved reductions of AFB 1 (13.53–35.53%) and AFM 1 (17.65–71.52%). Comparing with the positive control, the AFB 1 bioaccessibility ranged from 23.68 to 72.67% and for AFM 1 was 0%. The probiotic, isolated or combined with prebiotics, was efficient in mycotoxin reduction, while the combination of the two reduced the mycotoxin bioaccessibility. The best experimental condition was the highest concentration of AFB 1 (6.5 μg L −1 ) and AFM 1 (2.0 μg L −1 ), incubation time of 0 h and the addition of probiotic and inulin (0.75%).