Nuclear and chloroplast DNA phylogeography reveals P leistocene divergence and subsequent secondary contact of two genetic lineages of the tropical rainforest tree species S horea leprosula ( D ipterocarpaceae) in S outh‐ E ast A sia

• Published in: Molecular ecology 2013-04, Vol.22 (8), p.2264-2279
• Main Authors: Ohtani, Masato, Kondo, Toshiaki, Tani, Naoki, Ueno, Saneyoshi, Lee, Leong S., Ng, Kevin K. S., Muhammad, Norwati, Finkeldey, Reiner, Na'iem, Mohamad, Indrioko, Sapto, Kamiya, Koichi, Harada, Ko, Diway, Bibian, Khoo, Eyen, Kawamura, Kensuke, Tsumura, Yoshihiko
• Format: Article
• Language: English
• ISSN: 0962-1083; 1365-294X
• DOI: 10.1111/mec.12243

Tropical rainforests in S outh‐ E ast A sia have been affected by climatic fluctuations during past glacial eras. To examine how the accompanying changes in land areas and temperature have affected the genetic properties of rainforest trees in the region, we investigated the phylogeographic patterns of a widespread dipterocarp species, Shorea leprosula . Two types of DNA markers were used: expressed sequence tag‐based simple sequence repeats and chloroplast DNA (cp DNA ) sequence variations. Both sets of markers revealed clear genetic differentiation between populations in B orneo and those in the M alay P eninsula and S umatra ( M alay/ S umatra). However, in the south‐western part of B orneo, genetic admixture of the lineages was observed in the two marker types. Coalescent simulation based on cp DNA sequence variation suggested that the two lineages arose 0.28–0.09 million years before present and that following their divergence migration from Malay/Sumatra to Borneo strongly exceeded migration in the opposite direction. We conclude that the genetic structure of S . leprosula was largely formed during the middle P leistocene and was subsequently modified by eastward migration across the subaerially exposed S unda S helf.