Unique genome replication mechanism of the archaeal virus AFV 1

The exceptional genomic content and genome organization of the A cidianus filamentous virus 1 ( AFV 1) that infects the hyperthermophilic archaeon A cidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. An analysis of replicative intermediates of the v...

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Bibliographic Details
Published in:Molecular microbiology 2014-06, Vol.92 (6), p.1313-1325
Main Authors: Pina, Mery, Basta, Tamara, Quax, Tessa E. F., Joubert, Alexandra, Baconnais, Sonia, Cortez, Diego, Lambert, Sarah, Le Cam, Eric, Bell, Stephen D., Forterre, Patrick, Prangishvili, David
Format: Article
Language:English
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Summary:The exceptional genomic content and genome organization of the A cidianus filamentous virus 1 ( AFV 1) that infects the hyperthermophilic archaeon A cidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. An analysis of replicative intermediates of the viral genome by two‐dimensional (2 D ) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a D ‐loop and proceeds via strand displacement replication. Characterization of replicative intermediates using dark‐field electron microscopy, in combination with the 2 D agarose gel electrophoresis data, suggests that recombination plays a key role in the termination of AFV 1 genome replication through the formation of terminal loops. A terminal protein was found to be attached to the ends of the viral genome. The results allow us to postulate a model of genome replication that relies on recombination events for initiation and termination.
ISSN:0950-382X
1365-2958
DOI:10.1111/mmi.12630