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Two closely related members of A rabidopsis 13‐lipoxygenases (13‐ LOXs ), LOX3 and LOX4 , reveal distinct functions in response to plant‐parasitic nematode infection
The responses of two closely related members of A rabidopsis 13‐lipoxygenases (13‐ LOX s), LOX3 and LOX4 , to infection by the sedentary nematodes root‐knot nematode ( M eloidogyne javanica ) and cyst nematode ( H eterodera schachtii ) were analysed in transgenic A rabidopsis seedlings. The tissue l...
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Published in: | Molecular plant pathology 2014-05, Vol.15 (4), p.319-332 |
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Main Authors: | , , , , , , , , |
Format: | Article |
Language: | English |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | The responses of two closely related members of
A
rabidopsis
13‐lipoxygenases (13‐
LOX
s),
LOX3
and
LOX4
, to infection by the sedentary nematodes root‐knot nematode (
M
eloidogyne javanica
) and cyst nematode (
H
eterodera schachtii
) were analysed in transgenic
A
rabidopsis
seedlings. The tissue localization of
LOX3
and
LOX4
gene expression using β‐glucuronidase (
GUS
) reporter gene constructs showed local induction of
LOX3
expression when second‐stage juveniles reached the vascular bundle and during the early stages of plant–nematode interaction through gall and syncytia formation. Thin sections of nematode‐infested knots indicated
LOX3
expression in mature giant cells, and high expression in neighbouring cells and those surrounding the female body.
LOX4
promoter was also activated by nematode infection, although the
GUS
signal weakened as infection and disease progressed. Homozygous insertion mutants lacking
LOX3
were less susceptible than wild‐type plants to root‐knot nematode infection, as reflected by a decrease in female counts. Conversely, deficiency in
LOX4
function led to a marked increase in females and egg mass number and in the female to male ratio of
M
. javanica
and
H
. schachtii
, respectively. The susceptibility of
lox4
mutants was accompanied by increased expression of allene oxide synthase, allene oxide cyclase and ethylene‐responsive transcription factor 4, and the accumulation of jasmonic acid, measured in the roots of
lox4
mutants. This response was not found in
lox3
mutants. Taken together, our results reveal that
LOX4
and
LOX3
interfere differentially with distinct metabolic and signalling pathways, and that
LOX4
plays a major role in controlling plant defence against nematode infection. |
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ISSN: | 1464-6722 1364-3703 |
DOI: | 10.1111/mpp.12094 |