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Elicitation of hypersensitive responses in N icotiana glutinosa by the suppressor of RNA silencing protein P 0 from poleroviruses
Plant disease resistance ( R ) proteins that confer resistance to viruses recognize viral gene products with diverse functions, including viral suppressors of RNA silencing ( VSRs ). The P 0 protein from poleroviruses is a VSR that targets the ARGONAUTE 1 ( AGO 1) protein for degradation, thereby di...
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| Published in: | Molecular plant pathology 2015-06, Vol.16 (5), p.435-448 |
|---|---|
| Main Authors: | , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | Plant disease resistance (
R
) proteins that confer resistance to viruses recognize viral gene products with diverse functions, including viral suppressors of
RNA
silencing (
VSRs
). The
P
0 protein from poleroviruses is a
VSR
that targets the
ARGONAUTE
1 (
AGO
1) protein for degradation, thereby disrupting
RNA
silencing and antiviral defences. Here, we report resistance against poleroviruses in
N
icotiana glutinosa
directed against
T
urnip yellows virus
(
TuYV
) and
P
otato leafroll virus
(
PLRV
). The
P
0 proteins from
TuYV
(
P
0
T
u
),
PLRV
(
P
0
PL
) and
C
ucurbit aphid‐borne yellows virus
(
P
0
CA
) were found to elicit a hypersensitive response (
HR
) in
N
. glutinosa
accession
TW
59, whereas other accessions recognized
P
0
PL
only. Genetic analysis showed that recognition of
P
0
T
u
by a resistance gene designated
RPO
1
(
R
esistance to
PO
leroviruses
1
) is inherited as a dominant allele. Expression of
P
0 from a
P
otato virus
X
(
PVX
) expression vector transferred recognition to the recombinant virus on plants expressing
RPO
1
, supporting
P
0 as the unique
P
olerovirus
factor eliciting resistance. The induction of
HR
required a functional
P
0 protein, as
P
0
T
u
mutants with substitutions in the
F
‐box motif that abolished
VSR
activity were unable to elicit
HR
. We surmised that the broad
P
0 recognition seen in
TW
59 and the requirement for the
F
‐box protein motif could indicate detection of
P
0‐induced
AGO
1 degradation and disruption of
RNA
silencing; however, other viral silencing suppressors, including the
PVX P
25 that also causes
AGO
1 degradation, failed to elicit
HR
in
N
. glutinosa
. Investigation of
P
0 elicitation of
RPO
1 could provide insight into
P
0 activities within the cell that trigger resistance. |
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| ISSN: | 1464-6722 1364-3703 |
| DOI: | 10.1111/mpp.12201 |