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Quantitative trait loci mapping and transcriptome analysis reveal candidate genes regulating the response to ozone in A rabidopsis thaliana
As multifaceted molecules, reactive oxygen species ( ROS ) are known to accumulate in response to various stresses. Ozone ( O 3 ) is an air pollutant with detrimental effect on plants and O 3 can also be used as a tool to study the role of ROS in signalling. Genetic variation of O 3 sensitivity in d...
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| Published in: | Plant, cell and environment cell and environment, 2015-07, Vol.38 (7), p.1418-1433 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | As multifaceted molecules, reactive oxygen species (
ROS
) are known to accumulate in response to various stresses. Ozone (
O
3
) is an air pollutant with detrimental effect on plants and
O
3
can also be used as a tool to study the role of
ROS
in signalling. Genetic variation of
O
3
sensitivity in different
A
rabidopsis accessions highlights the complex genetic architecture of plant responses to
ROS
. To investigate the genetic basis of
O
3
sensitivity, a recombinant inbred line (
RIL
) population between two
A
rabidopsis accessions with distinct
O
3
sensitivity,
C
24 (
O
3
tolerant) and
T
e (
O
3
sensitive) was used for quantitative trait loci (
QTL
) mapping. Through analysis of
QTL
mapping combined with transcriptome changes in response to
O
3
, we identified three causal
QTLs
and several potential candidate genes regulating the response to
O
3
. Based on gene expression data, water loss and stomatal conductance measurement, we found that a combination of relatively low stomatal conductance and constitutive activation of salicylic acid (
SA
)‐mediated defence signalling were responsible for the
O
3
tolerance in
C
24. Application of exogenous
SA
prior to
O
3
exposure can mimic the constitutive
SA
signalling in
C
24 and could attenuate
O
3
‐induced leaf damage in the sensitive
A
rabidopsis accessions
T
e and
C
vi‐0.
Reactive oxygen species (
ROS
) are essential signaling molecules in plants that are also involved in the regulation of cell death. Genes that regulate
ROS
‐induced cell death were identified through
QTL
mapping and transcriptome analysis in ozone treated Arabidopsis accessions
C
24 and
Te
. A crucial protective role for the hormone salicylic acid was identified. |
|---|---|
| ISSN: | 0140-7791 1365-3040 |
| DOI: | 10.1111/pce.12499 |