Measures of cardiac function in T heraphosidae spiders using in vivo magnetic resonance imaging

We report the first in vivo cardiac magnetic resonance imaging (MRI) measurements of Theraphosidae spiders. MRI scanning is performed on six spiders under isoflurane‐induced anaesthesia. Retrospective self‐gating cine‐cardiac MRI (RG‐CINE‐MRI) is used to overcome the difficulties of prospective card...

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Bibliographic Details
Published in:Physiological entomology 2018-09, Vol.43 (3), p.207-213
Main Authors: Merrifield, Gavin D., Brydges, Nichola M., Hall, Lynsey, Mullin, James, Gallagher, Lindsay, Pizzi, Romain, Holmes, William M.
Format: Article
Language:English
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Summary:We report the first in vivo cardiac magnetic resonance imaging (MRI) measurements of Theraphosidae spiders. MRI scanning is performed on six spiders under isoflurane‐induced anaesthesia. Retrospective self‐gating cine‐cardiac MRI (RG‐CINE‐MRI) is used to overcome the difficulties of prospective cardiac gating in this species. The resulting RG‐CINE‐MRI images are successfully analyzed to obtain functional cardiac parameters from live spiders at rest. Cardiac ejection fraction is found to increase with animal mass (Pearson correlation 0.849, P  = 0.03) at a faster rate than myocardial tissue volume, whereas heart rate remains constant across animals. This suggests the spider heart undergoes additional biomechanical loading with age. The results of the present study demonstrate the potential for retrospective gating with respect to evaluating aspects of cardiac function in a wide range of previously inaccessible species.
ISSN:0307-6962
1365-3032
DOI:10.1111/phen.12245