Identification of phenotypic markers of B cells from patients with C hagas disease

• Published in: Parasite immunology 2013-07, Vol.35 (7-8), p.214-223
• Main Authors: Fares, R. C. G., Correa‐Oliveira, R., de Araújo, F. F., Keesen, T. S. L., Chaves, A. T., Fiuza, J. A., Ferreira, K. S., Rocha, M. O. C., Gomes, J. A. S.
• Format: Article
• Language: English
• ISSN: 0141-9838; 1365-3024
• DOI: 10.1111/pim.12038

Chagas disease was discovered more than a hundred years ago, but its pathogenesis is still not completely understood. Autoimmunity is one of the mechanisms shown to contribute to its pathogenesis, which may indicate an important participation of B lymphocytes. Patients with C hagas disease have shown increased percentage of B cells producing IL ‐10. However, there are no reports of the phenotypic markers of B cells producing IL ‐10 in patients with C hagas disease. For the first time in the literature, we evaluated the phenotypic profile of distinct markers of B cells from peripheral blood of noninfected individuals and patients with C hagas disease. Our results showed that patients with C hagas disease had a higher expression of CD 21 and CD 24 on the surface of CD 19 + B cells, while CD 43 and CD 23 were expressed equally in all groups. Moreover, the expression of MHC ‐ II ( HLA ‐ DR ), CD 80, CD 86, caspase‐3, granzyme B and intracellular IL ‐10 and TGF ‐β by CD 19 + B cells was higher in patients with C hagas disease. The results of IL ‐10 production within CD 19 + CD 5 + CD 1d + B cells showed a higher percentage of this cytokine in patients with C hagas disease. Thus, our data bring a new knowledge about distinct markers of B cells in immune responses of C hagas disease.