Akaline, saline and mixed saline–alkaline stresses induce physiological and morpho‐anatomical changes in L otus tenuis shoots

• Published in: Plant biology (Stuttgart, Germany) Germany), 2014-11, Vol.16 (6), p.1042-1049
• Main Authors: Paz, R. C., Reinoso, H., Espasandin, F. D., González Antivilo, F. A., Sansberro, P. A., Rocco, R. A., Ruiz, O. A., Menéndez, A. B.
• Format: Article
• Language: English
• ISSN: 1435-8603; 1438-8677
• DOI: 10.1111/plb.12156

Saline, alkaline and mixed saline–alkaline conditions frequently co‐occur in soil. In this work, we compared these plant stress sources on the legume L otus tenuis , regarding their effects on shoot growth and leaf and stem anatomy. In addition, we aimed to gain insight on the plant physiological status of stressed plants. We performed pot experiments with four treatments: control without salt (p H  = 5.8; EC  = 1.2 dS·m −1 ) and three stress conditions, saline (100 m m N a C l, p H  = 5.8; EC  = 11.0 dS·m −1 ), alkaline (10 m m N a HCO 3 , p H  = 8.0, EC  = 1.9 dS·m −1 ) and mixed salt–alkaline (10 m m N a HCO 3  + 100 m m N a C l, p H  = 8.0, EC  = 11.0 dS·m −1 ). Neutral and alkaline salts produced a similar level of growth inhibition on L . tenuis shoots, whereas their mixture exacerbated their detrimental effects. Our results showed that none of the analysed morpho‐anatomical parameters categorically differentiated one stress from the other. However, N a C l‐ and N a HCO 3 ‐derived stress could be discriminated to different extents and/or directions of changes in some of the anatomical traits. For example, alkalinity led to increased stomatal opening, unlike N a C l‐treated plants, where a reduction in stomatal aperture was observed. Similarly, plants from the mixed saline–alkaline treatment characteristically lacked palisade mesophyll in their leaves. The stem cross‐section and vessel areas, as well as the number of vascular bundles in the sectioned stem were reduced in all treatments. A rise in the number of vessel elements in the xylem was recorded in N a C l‐treated plants, but not in those treated exclusively with N a HCO 3.