The A rabidopsis oligopeptidases TOP 1 and TOP 2 are salicylic acid targets that modulate SA ‐mediated signaling and the immune response

Salicylic acid ( SA ) is a small phenolic molecule with hormonal properties, and is an essential component of the immune response. SA exerts its functions by interacting with protein targets; however, the specific cellular components modulated by SA and critical for immune signal transduction are la...

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Published in:The Plant journal : for cell and molecular biology 2013-11, Vol.76 (4), p.603-614
Main Authors: Moreau, Magali, Westlake, Timothy, Zampogna, Giulio, Popescu, George, Tian, Miaoying, Noutsos, Christos, Popescu, Sorina
Format: Article
Language:English
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Summary:Salicylic acid ( SA ) is a small phenolic molecule with hormonal properties, and is an essential component of the immune response. SA exerts its functions by interacting with protein targets; however, the specific cellular components modulated by SA and critical for immune signal transduction are largely unknown. To uncover cellular activities targeted by SA , we probed Arabidopsis protein microarrays with a functional analog of SA. We demonstrate that thimet oligopeptidases ( TOP s) constitute a class of SA ‐binding enzymes. Biochemical evidence demonstrated that SA interacts with TOP s and inhibits their peptidase activities to various degrees both in vitro and in plant extracts. Functional characterization of mutants with altered TOP expression indicated that TOP 1 and TOP 2 mediate SA ‐dependent signaling and are necessary for the immune response to avirulent pathogens. Our results support a model whereby TOP 1 and TOP 2 act in separate pathways to modulate SA ‐mediated cellular processes.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.12320