A multi‐colour/multi‐affinity marker set to visualize phosphoinositide dynamics in A rabidopsis

• Published in: The Plant journal : for cell and molecular biology 2014-01, Vol.77 (2), p.322-337
• Main Authors: Simon, Mathilde Laetitia Audrey, Platre, Matthieu Pierre, Assil, Sonia, van Wijk, Ringo, Chen, William Yawei, Chory, Joanne, Dreux, Marlène, Munnik, Teun, Jaillais, Yvon
• Format: Article
• Language: English
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/tpj.12358

Phosphatidylinositolphosphates ( PIP s) are phospholipids that contain a phosphorylated inositol head group. PIP s represent a minor fraction of total phospholipids, but are involved in many regulatory processes, such as cell signalling and intracellular trafficking. Membrane compartments are enriched or depleted in specific PIP s, providing a unique composition for these compartments and contributing to their identity. The precise subcellular localization and dynamics of most PIP species is not fully understood in plants. Here, we designed genetically encoded biosensors with distinct relative affinities and expressed them stably in A rabidopsis thaliana . Analysis of this multi‐affinity ‘ PIP line’ marker set revealed previously unrecognized localization of various PIP s in root epidermis. Notably, we found that PI (4,5)P 2 is able to localize PIP 2 ‐interacting protein domains to the plasma membrane in non‐stressed root epidermal cells. Our analysis further revealed that there is a gradient of PI 4P, with the highest concentration at the plasma membrane, intermediate concentration in post‐Golgi/endosomal compartments, and the lowest concentration in the Golgi. Finally, we also found a similar gradient of PI 3P from high in late endosomes to low in the tonoplast. Our library extends the range of available PIP biosensors, and will allow rapid progress in our understanding of PIP dynamics in plants.