Prospective randomized controlled blinded clinical trial evaluating biomarkers of acute kidney injury following 6% hydroxyethyl starch 130/0.4 or Hartmann's solution in dogs

• Published in: Journal of veterinary emergency and critical care (San Antonio, Tex. : 2000) Tex. : 2000), 2021-05, Vol.31 (3), p.306-314
• Main Authors: Boyd, Corrin J., Sharp, Claire R., Claus, Melissa A., Raisis, Anthea L., Hosgood, Giselle, Smart, Lisa
• Format: Article
• Language: English
• Subjects: clusterin; cystatin C; fluid therapy; kidney injury molecule‐1; neutrophil gelatinase‐associated lipocalin; osteopontin
• ISSN: 1479-3261; 1476-4431
• DOI: 10.1111/vec.13056

Objective: To evaluate the effect of 6% hydroxyethyl starch (HES) 130/0.4, compared with a Hartmann's solution control (CRYST), on urine biomarkers of acute kidney injury (AKI) in dogs prescribed a fluid bolus. Design: Randomized, controlled, blinded clinical trial January 2018 to February 2019. Setting: University teaching hospital. Animals: Forty client‐owned dogs. Interventions: Dogs prescribed a fluid bolus were randomized to receive at least 10 mL/kg of HES or CRYST with clinicians and investigators blinded to fluid type. Study fluid was used for further boluses as required in the following 24 hours, to a limit of 40 mL/kg total, after which fluid administration was open‐label. Measurements and Main Results: Urine was collected prior to and 6, 12, and 24 hours after the first study fluid bolus. Urine concentrations of AKI biomarkers: neutrophil gelatinase‐associated lipocalin (NGAL), cystatin C, kidney injury molecule‐1 (KIM), clusterin, and osteopontin were measured using a magnetic bead multiplexed assay. Osmolality‐indexed biomarker concentrations were compared between groups over time with linear mixed‐effects models, with P