Effect of polyphenolic compounds on the renal Na+,K+-ATPase during the restoration of normotension after experimentally induced hypertension in rats

• Published in: Experimental physiology 2003-07, Vol.88 (4), p.475-482
• Main Authors: Javorková, Veronika, Pechánová, Olga, Andriantsitohaina, Ramaroson, Vrbjar, Norbert
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Animals; Blood Pressure - drug effects; Enzyme Inhibitors; Flavonoids - pharmacology; Full Length Papers; Hypertension - chemically induced; Hypertension - drug therapy; Hypertension - metabolism; Kidney - enzymology; Kinetics; Male; NG-Nitroarginine Methyl Ester; Nitric Oxide Synthase - metabolism; Phenols - pharmacology; Polyphenols; Rats; Rats, Inbred WKY; Sodium-Potassium-Exchanging ATPase - metabolism
• ISSN: 0958-0670; 1469-445X
• DOI: 10.1113/eph8802553

It is commonly known that consumption of foods and beverages rich in polyphenols is associated with a lower incidence of cardiovascular disease. The purpose of this study was to assess whether the application of red wine polyphenols influences the kinetic properties of renal Na+,K+-ATPase in rats in which hypertension has been experimentally induced by the nitric oxide synthase inhibitor L-NAME. Treatment with polyphenols during the recovery from hypertension to normotension resulted in the complete revival of the functional properties of the Na+,K+-ATPase, as indicated by the total restoration of Km, KNa (concentration of Na+ necessary to achieve half-maximal reaction velocity) and Vmax for enzyme activation by ATP and/or Na+ to pre-hypertension values. Two positive effects of polyphenols during the recovery period are indicated: a restoration of the affinity of the ATP and Na+ binding sites to control values and a probable increase in the number of Na+,K+-ATPase molecules to a level comparable to that in control conditions, as suggested by the complete renewal of Vmax. Experimental Physiology (2003) 88.4, 475-482.