SU-G-TeP3-10: Radiation Induces Prompt Live-Cell Metabolic Fluxes

• Published in: Medical physics (Lancaster) 2016-06, Vol.43 (6), p.3676-3676
• Main Authors: Campos, D, Peeters, W, Nickel, K, Burkel, B, Bussink, J, Kimple, R, van der Kogel, A, Eliceiri, K, Kissick, M
• Format: Article
• Language: English
• Subjects: 60 APPLIED LIFE SCIENCES; Cancer; Enzymes; Fluorescence; FLUORINE COMPOUNDS; GLYCOLYSIS; Intracellular transport; NEOPLASMS; PARTICLE TRACKS; RADIATION MONITORING; RADIATION MONITORS; RADIATION PROTECTION AND DOSIMETRY; Radiation therapy; Radiation treatment
• ISSN: 0094-2405; 2473-4209
• DOI: 10.1118/1.4957090

Purpose: To compare metabolic dynamics and HIF-1α expression following radiation between a cancerous cell line (UM-SCC-22B) and a normal, immortalized cell line, NOK (Normal Oral Keratinocyte). HIF-1 is a key factor in metabolism and radiosensitivity. A better understanding of how radiation affects the interplay of metabolism and HIF-1 might give a better understanding of the mechanisms responsible for radiosensitivity. Methods: Changes in cellular metabolism in response to radiation are tracked by fluorescence lifetime of NADH. Expression of HIF-1α was measured by immunofluorescence for both cell lines with and without irradiation. Radiation response is also monitored with additional treatment of a HIF-1α inhibitor (chrysin) as well as a radical scavenger (glutathione). Changes in oxygen consumption and respiratory capacity are also monitored using the Seahorse XF analyzer. Results: An increase in HIF-1α was found to be in response to radiation for the cancer cell line, but not the normal cell line. Radiation was found to shift metabolism toward glycolytic pathways in cancer cells as measured by oxygen consumption and respiratory capacity. Radiation response was found to be muted by addition of glutathione to cell media. HIF-1α inhibition similarly muted radiation response in cancer. Conclusion: The HIF-1 protein complex is a key regulator cellular metabolism through the regulation of glycolysis and glucose transport enzymes. Moreover, HIF-1 has shown radio-protective effects in tumor vascular endothelia, and has been implicated in metastatic aggression. Monitoring interplay between metabolism and the HIF-1 protein complex can give a more fundamental understanding of radiotherapy response.