Molecular and Functional Characterization of N -Acetyltransferases in Common Marmosets and Pigs

• Published in: Drug metabolism and disposition 2022-11, Vol.50 (11), p.1429-1433
• Main Authors: Uno, Yasuhiro, Uehara, Shotaro, Ijiri, Moe, Kawaguchi, Hiroaki, Asano, Atsushi, Shiraishi, Mitsuya, Banju, Kaito, Murayama, Norie, Yamazaki, Hiroshi
• Format: Article
• Language: English
• Subjects: 4-Aminobenzoic Acid - metabolism; Acetyltransferases - genetics; Animals; Arylamine N-Acetyltransferase - genetics; Arylamine N-Acetyltransferase - metabolism; Callithrix - metabolism; Fluorenes; Humans; Isoniazid - metabolism; Mice; Phylogeny; Rats; Recombinant Proteins - metabolism; Sulfamethazine; Swine
• ISSN: 0090-9556; 1521-009X
• DOI: 10.1124/dmd.122.000919

Arylamine -acetyltransferases (NATs) are drug-metabolizing enzymes that are essential for the metabolism of endogenous substrates and xenobiotics. The molecular characteristics of NATs have been extensively investigated in humans but remain to be investigated in common marmosets and pigs, animal species that are often used in drug metabolism studies. In this study, marmoset NAT1 and pig NAT1 cDNAs were isolated from liver samples and were characterized by molecular analyses and drug-metabolism assays. These genes were intronless and formed gene clusters with one other gene in the genome, just as human genes do. Marmoset NAT1 and pig NAT1 amino acid sequences showed high sequence identities (94% and 85%, respectively) to human NAT1. Phylogenetic analysis indicated that marmoset NAT1 and pig NAT1 were more closely clustered with human NATs than with rat or mouse NATs. Marmoset NAT1 and pig NAT1 mRNAs were expressed in all the tissue types analyzed, with the expression levels being highest in the small intestine. Metabolic assays using recombinant proteins found that marmoset NAT1 and pig NAT1 metabolized human NAT substrates -aminobenzoic acid, 2-aminofluorene, sulfamethazine, and isoniazid. Marmoset NAT1 and pig NAT1 substantially acetylated -aminobenzoic acid and 2-aminofluorene relevant human NAT1, but their activities were lower toward sulfamethazine and isoniazid than those of the relevant human NAT2. Therefore, marmoset and pig NATs are functional enzymes with molecular similarities to human NAT1, but their substrate specificities, while similar to human NAT1, differ somewhat from human NAT2. SIGNIFICANCE STATEMENT: Marmoset -acetyltransferase NAT1 and pig NAT1 were identified and showed high sequence identities to human NAT1. These NAT mRNAs were expressed in various tissues. Marmoset and pig NAT1s acetylated typical human NAT substrates, although their substrate specificities differed somewhat from human NAT2. Marmoset NAT1 and pig NAT1 have similarities with human NAT1 in terms of molecular and enzymatic characteristics.