Axenic Cultivation of Entamoeba histolytica

Entamoeba histolytica (National Institutes of Health strain 200) has been maintained in axenic culture through 50 transfers over a period of 6 months. The medium used is diphasic and contains a cell-free extract of chick embryo which is essential for growth. Inocula of 50,000 amoebas yield 150,000 t...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1961-08, Vol.134 (3475), p.336-337
Main Author: Diamond, Louis S.
Format: Article
Language:English
Subjects:
Amoeba
Axenic culture
Broths
Depolarization
Embryonic cells
Embryos
Parasitism
Refrigerators
Room temperature
Spinal Nerve roots
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Entamoeba histolytica (National Institutes of Health strain 200) has been maintained in axenic culture through 50 transfers over a period of 6 months. The medium used is diphasic and contains a cell-free extract of chick embryo which is essential for growth. Inocula of 50,000 amoebas yield 150,000 to 200,000 organisms in 72 hours of incubation at 35° C. An extract prepared from boiled embryos supports growth equally well.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.134.3475.336