Antibodies to Photoproducts of Deoxyribonucleic Acids Irradiated with Ultraviolet Light

A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. Serologic activity appeared after irradiation of DNA at 270 mμ and decreased upon irradiation at 235 mμ. The antigeni...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1966-09, Vol.153 (3744), p.1666-1667
Main Authors: Levine, Lawrence, Seaman, Edna, Hammerschlag, Ellen, van Vunakis, Helen
Format: Article
Language:English
Subjects:
Albumins
Animals
Antibodies
Antibody Formation
Antigen-Antibody Reactions
Antiserum
Dimers
DNA
DNA, Bacterial
Epitopes
Grants
Irradiation
Light
Nucleotides
Proteus - immunology
Proteus - radiation effects
Rabbits
Radiation Effects
Serum Albumin, Bovine
Ultraviolet radiation
Ultraviolet Rays
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Description
Summary:A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. Serologic activity appeared after irradiation of DNA at 270 mμ and decreased upon irradiation at 235 mμ. The antigenic determinants of the ultraviolet-treated DNA appear to be photoproducts associated primarily with thymine, as measured by direct dependence of serologic activity on the adenine-thymine content of the DNA, and by inhibition of the serologic reaction by the irradiated di-, tri-, and tetra-(thymidine-5′-phosphate) nucleotides.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.153.3744.1666