Antibodies to Photoproducts of Deoxyribonucleic Acids Irradiated with Ultraviolet Light

A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. Serologic activity appeared after irradiation of DNA at 270 mμ and decreased upon irradiation at 235 mμ. The antigeni...

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Published in:Science (American Association for the Advancement of Science) 1966-09, Vol.153 (3744), p.1666-1667
Main Authors: Levine, Lawrence, Seaman, Edna, Hammerschlag, Ellen, van Vunakis, Helen
Format: Article
Language:English
Subjects:
Albumins
Animals
Antibodies
Antibody Formation
Antigen-Antibody Reactions
Antiserum
Dimers
DNA
DNA, Bacterial
Epitopes
Grants
Irradiation
Light
Nucleotides
Proteus - immunology
Proteus - radiation effects
Rabbits
Radiation Effects
Serum Albumin, Bovine
Ultraviolet radiation
Ultraviolet Rays
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cited_by cdi_FETCH-LOGICAL-c381t-4f7c78f42469b4eb9b5f4643c47ff2644786cf75a2122c56c866ed6472da48d53
cites cdi_FETCH-LOGICAL-c381t-4f7c78f42469b4eb9b5f4643c47ff2644786cf75a2122c56c866ed6472da48d53
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container_issue 3744
container_start_page 1666
container_title Science (American Association for the Advancement of Science)
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creator Levine, Lawrence
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Hammerschlag, Ellen
van Vunakis, Helen
description A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. Serologic activity appeared after irradiation of DNA at 270 mμ and decreased upon irradiation at 235 mμ. The antigenic determinants of the ultraviolet-treated DNA appear to be photoproducts associated primarily with thymine, as measured by direct dependence of serologic activity on the adenine-thymine content of the DNA, and by inhibition of the serologic reaction by the irradiated di-, tri-, and tetra-(thymidine-5′-phosphate) nucleotides.
doi_str_mv 10.1126/science.153.3744.1666
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Serologic activity appeared after irradiation of DNA at 270 mμ and decreased upon irradiation at 235 mμ. The antigenic determinants of the ultraviolet-treated DNA appear to be photoproducts associated primarily with thymine, as measured by direct dependence of serologic activity on the adenine-thymine content of the DNA, and by inhibition of the serologic reaction by the irradiated di-, tri-, and tetra-(thymidine-5′-phosphate) nucleotides.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.153.3744.1666</identifier><identifier>PMID: 5917082</identifier><language>eng</language><publisher>United States: American Association for the Advancement of Science</publisher><subject>Albumins ; Animals ; Antibodies ; Antibody Formation ; Antigen-Antibody Reactions ; Antiserum ; Dimers ; DNA ; DNA, Bacterial ; Epitopes ; Grants ; Irradiation ; Light ; Nucleotides ; Proteus - immunology ; Proteus - radiation effects ; Rabbits ; Radiation Effects ; Serum Albumin, Bovine ; Ultraviolet radiation ; Ultraviolet Rays</subject><ispartof>Science (American Association for the Advancement of Science), 1966-09, Vol.153 (3744), p.1666-1667</ispartof><rights>Copyright 1966 American Association for the Advancement of Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-4f7c78f42469b4eb9b5f4643c47ff2644786cf75a2122c56c866ed6472da48d53</citedby><cites>FETCH-LOGICAL-c381t-4f7c78f42469b4eb9b5f4643c47ff2644786cf75a2122c56c866ed6472da48d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/1719817$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/1719817$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,2884,2885,27924,27925,58238,58471</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/5917082$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Levine, Lawrence</creatorcontrib><creatorcontrib>Seaman, Edna</creatorcontrib><creatorcontrib>Hammerschlag, Ellen</creatorcontrib><creatorcontrib>van Vunakis, Helen</creatorcontrib><title>Antibodies to Photoproducts of Deoxyribonucleic Acids Irradiated with Ultraviolet Light</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>A rabbit immunized with complexes of methylated bovine serum albumin and ultraviolet-irradiated DNA from calf thymus produced antibodies directed toward the photoproducts in the DNA. 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source American Association for the Advancement of Science; JSTOR Archival Journals and Primary Sources Collection
subjects Albumins
Animals
Antibodies
Antibody Formation
Antigen-Antibody Reactions
Antiserum
Dimers
DNA
DNA, Bacterial
Epitopes
Grants
Irradiation
Light
Nucleotides
Proteus - immunology
Proteus - radiation effects
Rabbits
Radiation Effects
Serum Albumin, Bovine
Ultraviolet radiation
Ultraviolet Rays
title Antibodies to Photoproducts of Deoxyribonucleic Acids Irradiated with Ultraviolet Light
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